npm

primer3

Designs PCR primers and hybridization probes for genomic DNA

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About

Program for designing PCR primers

Commands

primer3_coreprimer3_masker

Examples

Design primers for a DNA sequence from input file$ primer3_core < input.txt > output.txt
Design primers with custom primer length constraints$ primer3_core -default_version=2 < sequence_input.txt > primers.txt
Mask repetitive sequences before primer design$ primer3_masker -in sequence.fasta -out masked.fasta