kallisto

Quantify transcript abundances from RNA-Seq data using k-mer matching

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Quantify abundances of transcripts from RNA-Seq data

Commands

kallisto

Examples

Build a kallisto index from a reference transcriptome$ kallisto index -i transcripts.idx transcripts.fasta
Quantify abundances for paired-end RNA-Seq reads$ kallisto quant -i transcripts.idx -o output reads_1.fastq reads_2.fastq
Quantify single-end reads with bootstrap sampling for uncertainty estimation$ kallisto quant -i transcripts.idx -o output --single -l 200 -s 20 -b 100 reads.fastq