bbtools

Suite of tools for high-speed read processing and sequence analysis

brewmacoslinux
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Source

About

Brian Bushnell's tools for manipulating reads

Commands

bbduk.shbbmap.shbbnorm.shdedupe.shfilterbyname.shreformat.shstatkey.shtadpole.sh

Examples

Remove adapters and low-quality reads from FASTQ file$ bbduk.sh in=reads.fastq out=cleaned.fastq ref=adapters.fa ktrim=r k=23 mink=11 hdist=1 tpe tbo
Map reads to reference genome and generate BAM output$ bbmap.sh ref=reference.fasta in=reads.fastq out=mapped.sam
Normalize read depth to target coverage$ bbnorm.sh in=reads.fastq out=normalized.fastq target=100 mindepth=5